| Using culture
systems consisting of osteoblastic cells and vascular
endothelial cells, our investigations have demonstrated that
Endothelin-1, a vascular polypeptide, stimulates osteoblast
differentiation and bone production. This effect is additive to
the known stimulatory effects of the steroid Dexamethasone, and
our microarray experiments have shown that the two factors
control osteoblastic cells along common pathways, but also
stimulate these cells by unique mechanisms. Present
investigations are looking at the roles of VEGF and CBFA1 in
this system.
Active collaborations in within our lab
with Dr. Heersche and Dr. Manolson are ongoing. We are also
collaborating with Dr. Dennis Cvitkovitch and Dr. Yung-Hua Li on
the study of Biofilms on orthopaedic implants.
My clinical practice as a Hand Surgeon
focuses on the diseases and trauma that affect the hands.
Osteoporotic fractures, avascular necrosis of bone and fracture
non-unions are conditions that we frequently see in the hand.
Investigating the biology of bone in the laboratory will help us
to develop new strategies to deal with these conditions.
Visit the Bone Lab www.utoronto.ca/bonelab
for more information
Related Publications:
Veillette CJ, von Schroeder HP.
Endothelin-1 down-regulates the expression of vascular endothelial growth
factor-A associated with osteoprogenitor proliferation and
differentiation.
Bone. 2004 Feb;34(2):288-96.
von Schroeder HP, Veillette CJ, Payandeh J, Qureshi A, Heersche
JN.
Endothelin-1 promotes osteoprogenitor proliferation and differentiation in
fetal rat calvarial cell cultures.
Bone. 2003 Oct;33(4):673-84.
Billings E Jr,
von Schroeder HP, Mai M, Aratow M, Amiel D, Woo SL-Y, Coutts RD:
Cartilage resurfacing of the rabbit knee: The use of an
allogeneic demineralized bone matrix-autogenic perichondrium
composite implant. Acta Orthopaedic Scandinavia 1990: 61: pp
201.
von Schroeder HP,
Kwan M, Amiel D, Coutts RD: The use of polylactic acid matrix
and periosteal grafts for the reconstruction of rabbit knee
articular defects. Journal of Biomaterials Research 1991: 25: pp
329-339.
Coutts RD, Woo
SL-Y, Amiel D, von Schroeder HP, Kwan MK: Rib perichondrial
autografts in full-thickness articular cartilage defects in
rabbits. Clinical Orthopaedics and Related Research 1992: 275:
pp 263-273.
von Schroeder HP,
Hashimoto Y, Heersche JNM: The effects of natural and synthetic
retinoids on the differentiation of RCJ C5.18 chondrogenic
cells. Teratology 1994: 50: pp 54-62.
von Schroeder HP,
Miura Y, Fitzsimmons J, Commisso C, O'Driscoll SW: A comparison
of the chondrogenic potentials of periosteum and perichondrium
in vitro. Orthopaedic Transactions 1994: 18(2): pp 468.
von Schroeder HP,
Heersche JNM: Retinoic acid-responsiveness of cells and tissues
in developing fetal limbs evaluated in a RAREhspLacZ transgenic
mouse model. Journal of Orthopaedic Research 1998: 16: pp
355-364.
von Schroeder HP, Chen X, Heersche JNM: Endothelin-1 stimulates
fetal rat osteoblastic progenitors to proliferate and form bone
nodules. J. Bone Min. Res. 2000: 15: pp S378. |
